Within biology, a isothiocyanate derivative of fluorescein is typically wont to label & track cells in fluorescence microscopy applications. A fluorescence of this molecule is very high, & excitation occurs at 494nm & emission at 525nm. A isothiocyanate class action (-North=C=S) replaces the hydrogen atom on the bottom ring of the structure, and is reactive using amine groups on proteins in cells. Fluorescein isothiocyanate is typically abbreviated when FITC. Extra biologically active molecules (like antibodies) may likewise exist as bonded to fluoresceine, allowing for even life scientist to target a fluorophore to specific proteins or structures in cells.

One of its extra recognizable utilizes is in the Chicago River, where fluorescent dye is utilized to dye a flow of any stream green on St. Patrick's Day.

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